CandrisDB

CanDriS is a software which uses an empirical Bayesian procedure to calculate the posterior probability of a site to be cancer-driving for all sites in a gene. To use it, please upload a mutation annotation format (maf) file that contains the somatic point mutations that are to be investigated. The file should contain the following four tab-delimited columns in the given order (including a header):

(1) Gene id

– corresponds to the official gene id as used in the Ensembl 75: Feb 2014(GRCh37.p13); for example, ENSG00000084734

(2) Transcript id

- the transcript which is most commonly used in TCGA, can be downloaded from this reference file; for example, ENST00000264717

(3) DNA mutation

– the allele change in the mutation site; for example, 1740C>A

(4) Protein mutation

– the amino acid change in the mutation site; for example, F580L

(5) Mutation count

– the mutation frequency in your own cohort

The example file:

gene_id	trancript_id	DNA_mutation	protein_mutation	mutation_count
ENSG00000084734	ENST00000264717	1740C>A	F580L	1
ENSG00000120669	ENST00000379881	389G>A	S130N	1
ENSG00000011258	ENST00000415868	547A>G	T183A	1
ENSG00000160716	ENST00000368476	758T>C	I253T	1
ENSG00000141867	ENST00000263377	3139C>T	R1047W	1

By default, you can run CanDriS just based on your own data if you have a big enough cohort or a unique tumor type samples differ from TCGA. Alternatively, you can also choose to run CanDriS by merging your sample into TCGA MC3 PANCAN or a specific tumor type data.

In the web page, we only display the sites with a posterior posibility over 0.5 for convenient. The complete result can be downloaded from the "Download" button at the bottom of the page.

Runtime depends on server usage and the size of the data set. Enter your e-mail address to receive the results when they are ready. Alternatively, you can keep the browser window open and check back later. It won't take a long time.